Biofilm formation by Mycobacterium avium isolates originating from humans, swine and birds

源自人类、猪和鸟类的鸟分枝杆菌分离株形成的生物膜

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作者:Tone Bjordal Johansen, Angelika Agdestein, Ingrid Olsen, Sigrun Fredsvold Nilsen, Gudmund Holstad, Berit Djønne

Background

Mycobacterium avium includes the subspecies avium, silvaticum, paratuberculosis and hominissuis, and M. avium subspecies has been isolated from various environments all over the world including from biofilms in water distribution systems. The

Conclusion

The observed differences in biofilm forming abilities between porcine and human isolates raises questions regarding the importance of biofilm formation for infectious potential. The optimised method worked well for screening of multiple isolates.

Results

Nine isolates from swine produced biofilm. There was a significant higher frequency of porcine isolates forming biofilm compared to human isolates. All isolates were previously characterised by IS1311- and IS1245-RFLP typing. The ability to form biofilm did not correlate with the RFLP-type, hsp65 sequevar, colony morphology or the presence of gene sequences related to GPL synthesis.

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