Abstract
The Butterfly hillstream loach (Beaufortia pingi), an aquatic benthic fish species inhabiting mountain rapids, exhibits exceptional capabilities in movement, adsorption, and desorption processes, enabling it to adhere to smooth and contaminated surfaces in turbulent streams. These attributes make it a significant subject for genetic and evolutionary research. In this study, the genomic sequences of this species were acquired utilizing PacBio sequencing and Hi-C methods. The genome assembly is 459.8 Mb in size with a contig N50 of 5.35 Mb, and the assembled contigs were anchored into 25 chromosomes. BUSCO analysis confirmed a high completeness level with 97.0% gene coverage. A total of 111.47 Mb repetitive sequences (24.25% of the assembled genome), and 22,906 protein-coding genes were identified in the genome. This study represents the first investigation of the species' genome. The establishment of this genome assembly provides valuable resources for future genetic research and facilitates the study of genetic changes during evolution.