Sodium Channel Isoform Diversity Underlies Chamber-Specific Cardiac Excitability.

BACKGROUND: Na(V) (voltage-gated sodium) channels drive cardiac excitability. Although Na(V)1.5 is the primary cardiac isoform, the composition and functional contributions of non-Na(V)1.5 isoforms in the heart remain unclear. METHODS: Here, we developed a chemical-genetic mouse model (Na(V)1.5(GX/GX)) in which Na(V)1.5 can be selectively and reversibly inhibited by acyl- and aryl-sulfonamide compounds (GX [acyl- and aryl-sulfonamide compounds typically denoted by the name GX-### and associated items] drugs). Cardiac activity was assessed by electrocardiograms in vivo, and optical mapping was used for imaging of ex vivo hearts. Whole-cell voltage-clamp in tandem with validated toxins and isoform-selective inhibitors were used to examine sodium current composition. RESULTS: Na(V)1.5(GX/GX) mice exhibited normal cardiac function at baseline, but acute GX drug administration caused profound conduction defects and arrhythmias. Whole-heart optical mapping revealed dose-dependent chamber-specific sensitivity to Na(V)1.5 inhibition, with the right ventricle being the most sensitive, followed by the left ventricle, left atrium, and right atrium. Patch-clamp recordings of isolated cardiomyocytes with application of Na(V) isoform-selective inhibitors showed that Na(V)1.5 contributed 93% of sodium current in the left ventricle, 79% in the right ventricle, and 78% in the atria. Non-Na(V)1.5 isoforms were differentially enriched across chambers: Na(V)1.8 in the left ventricle, Na(V)1.1/1.3 in the right ventricle, and Na(V)1.2/1.6/1.7 in the atria. CONCLUSIONS: These results reveal a surprising chamber-specific isoform landscape of cardiac sodium currents, which may underlie the right ventricular predominant phenotype of Brugada syndrome. These data highlight non-Na(V)1.5 isoforms as potential mediators of chamber-specific cardiac pathologies and as pharmacological targets.