Evaluation of Tegaran Formula ZhenHua cytotoxicity against human cancer cell lines

评价Tegaran Formula ZhenHua对人癌细胞系的细胞毒性

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作者:Panagiotis Parsonidis ,Ioanna Vlachou ,Alexandra Mamagkaki ,Ioannis Bouris ,Vasiliki Daikopoulou ,Ioannis Papasotiriou

Abstract

The aim of this study is to evaluate the potential health effects of Tegaran Formula ZhenHua, a nutritional supplement used mainly by cancer patients. Its active ingredients and cytotoxicity was assessed with analytical methods and viability assays, respectively. The analytical methods consisted of dissolution, disintegration, HPLC, LC/MS, GC/MS and NMR. Cytotoxicity was assessed by MTT, SRB, CVE colorimetric viability assays in 0, 24, 48 and 72h time points. The results indicate that Tegaran Formula ZhenHua supplement did not present any cytotoxic effects due to issues related to the capsules' solubility, distribution and identification of the active ingredient.

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