Abstract
Graphene exhibits remarkable properties and holds substantial promise for diverse applications. Its unique combination of thermal, chemical, physical, and biological properties makes it an appealing material for a wide range of uses. But, the lack of an economical and environmentally friendly approach to synthesize good-quality graphene represents an immense challenge for the scientific community. What makes this research unique is the utilization of the defensive gland extract from the beetle species Luprops tristis (Order: Coleoptera, Family: Tenebrionidae) to synthesize a few layers of graphene (FLG). This innovative incorporation of natural resources and exploration of biologically inspired methods provides an eco-friendly and cost-effective alternative to conventional graphene production techniques. The exfoliated graphene displayed antimicrobial effects against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, with particularly potent bactericidal activity. Additionally, the cytotoxicity assay demonstrated the anti-cancer properties of biosynthesized graphene against Daltons Lymphoma Acetic (DLA) cells.