Conclusion
cpTi exhibited the highest corrosion resistance in comparison to Ag-Pd and Ni-Cr alloys. Ag-Pd alloys showed acceptable resistance to corrosion that is due to the passivity effect. Also, artificial saliva increased the cytotoxic effect of the tested alloys more than DMEM.
Methods
The sample size used was 10 discs from each alloy used with dimensions of 4x3mm. The HGF was derived from healthy patients subjected to gingivectomy procedures. Of the specimens, 50% were incubated in artificial saliva and the other half in Dulbecco's Modified Eagle medium (DMEM). The extract of each alloy in both media was collected and applied on HGF. After 24 hours the morphology of the HGF cells was examined to detect any apoptosis or cell death. Also, cell viability was evaluated by the use of a 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Statistical analysis was performed using students' t-test and two-way ANOVA with a significance level of p<0.05.
Results
In the case of morphological examination of HGF and MTT assessment, only cpTi alloy specimens didn't display any cytotoxic effect. Ni-Cr was the most cytotoxic alloy of the three. Also, MTT activities of all three alloys were decreased when they were incubated in artificial saliva.