Abstract
The aim of this present study was to investigate the environmental proficiency of two laccase producing bacterial strains, Hb16c and Berl11b2. Here, laccases, which were secreted in media containing environmental wastes, were characterized for biochemical and kinetic novelty and applied in the decolourization of some synthetic dyes and subsequently, denim bleaching. The laccases exhibited enhanced pH-, thermo-, psychro-, metal-, halo-, and surfacto-tolerance, eliciting residual activities of at least ca. 71%. Thereafter, the enzymes were able to decolourize novel high concentrations of synthetic dyes (0.2% w v-1) at 56 h of incubation, and also elicit a mediator-assisted perpetual wash up and decolourization of indigo pigment from fabric under 6 h. The outcomes observed in this study therefore warrant the adoption of these isolates for applications toward a sustainable and total environment through production of fine biochemicals, and the minimization of environmental wastes.