Endometriotic cell culture contamination and authenticity: a source of bias in in vitro research?

子宫内膜异位细胞培养污染和真实性:体外研究偏见的来源?

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作者:Andrea Romano, Sofia Xanthoulea, Elisa Giacomini, Bert Delvoux, Eugenia Alleva, Paola Vigano

Methods

Literature review part: almost 300 published protocols describing the isolation and creation of primary cell cultures from endometriosis were reviewed. Wet-lab part: primary cells isolated from 13 endometriosis patients were analysed by immunohistochemistry, immunofluorescence and FACS for the expression of Pax2. Cell lines Z11 and Z12, the most diffused endometriosis cell lines, were characterised with respect to the expression of Pax2, steroid hormone receptors and STR profile. Main

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