MicroRNA-155 complementation on a chemically functionalized dual electrode surface for determining breast cancer

MicroRNA-155 在化学功能化的双电极表面互补用于乳腺癌检测

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作者:Subash C B Gopinath, Veeradasan Perumal, Shijin Xuan

Abstract

This study correlated and quantified the expression of microRNA-155 with breast cancer to determine breast cancer progression. The target microRNA-155 sequence was identified by complementation on a capture-probe sequence-immobilized interdigitated dual electrode surface. The sensitivity was found to be 1 fM, and the limit of detection fell between 1 and 10 fM. The specific sequence selectivity with single mismatches, triple mismatches, and noncomplementary bases failed to complement the capture-probe sequence. The obtained results demonstrate the selective determination of the microRNA-155 sequence and can help to diagnose breast cancer.

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