Inducible RNA Interference of brlAbeta in Aspergillus nidulans

构巢曲霉中brlAbeta的诱导RNA干扰

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作者:L M Barton, R A Prade

Abstract

An inducible RNA interference (RNAi) construct composed of inverted repeating alcA promoters flanking the developmental regulatory gene brlAbeta was tested in Aspergillus nidulans. On inducing medium, the RNAi strains failed to sporulate and lacked brlAalpha and brlAbeta expression. RNAi was specific for brlAbeta, but not brlAalpha, silencing, indicating brlAalpha regulation by brlAbeta.

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