Abstract
Horse oil-in-water (O/W) emulsions were prepared and α-tocopherol was added at 0, 100, 200, and 500 ppm (α-T0, α-T100, α-T200, α-T500) to enhance its oxidative stability. Mean particle diameters of the O/W emulsions were 243-299 nm. Zeta potential values increased with the addition of α-tocopherol; however, they decreased during storage at 40 °C for 30 days. Particle size distribution of the O/W emulsion with α-tocopherol remained the same as that of α-T0. For lipid oxidation, the peroxide values of α-T0 and α-T500 were greatly increased from 2.96 and 2.89 to 13.76 and 12.46 mmol/kg oil, respectively, after 30 days. The α-T100 and α-T200 maintained lower peroxide values than other emulsions. Thiobarbituric acid-reactive substance values of α-T0 and α-T500 were higher than those of α-T100 and α-T200. These results indicate that the addition of α-tocopherol from 100 to 200 ppm to the horse oil-in-water emulsion effectively improves its oxidative stability during storage.